Process to separate glutaminic acid from other amino acids.



i r o co or n 1- acres no car l NE a 11:1; QURICH, SWITZERL I t lit TlE@LUTAMINIC ACID FROM OTHER AMINO ACIDS.

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, To all whom it may concerni- Improvements in Processes to Separatelit) Glutaminic Acid from other Amino Acids; and l do hereby declare thefollowing to be a W clear, and exact description of the invention, suchas will enable others skilled in the art to which it appertains to makeand use the same.

As is already known, glutaminic acid, as well as a series of other aminoacids, is obtained in the most simple manner by hydrolysis of albuminswith mineral acids. lln the hydrolysate it is combined as a salt withthe solvent acid. i

en using HCl for the purpose of isolating the glutaminic acid, it washitherto usual to introduce hydrochloric acid gas into the hydrolysate,in order to convert into saturated hydrochloric acid the water which wasleft over from the hydrolysis and which dissolved the glutaminicacid-hydrochlorid; for by this saturation the hydrochlorid, which isinsoluble in saturated hydrochloric acid. is precipitated.

This as well as the other usual processes, have several disadvantagegforexample the escape of large quantities of hydrochloric acid gas and theproduction of undesirable motherlyes. Besides this process rendersnecessary the installation of a somewhat complicated plant for producinghydrochloric acid gas, which one has long wished to avoid. v

The process hereinafter described removes these isadvantages. As isknown, free glutanmnic acid dissolves hardly in water; its solubility is1:100. The glutaminic acid will therefore be separated from its solutionin the hydrolysate by getting rid of the H01, combined with it, as wellas of the superfiuous free H Cl, which hinders the glutaminicacid-hydrochlorid from crystallizin the diluted solution.

The process which is the subject of the present discovery may be carriedout with advantage as follows:

After the albumins, which are suitable for this purpose, preferablythose which give ll a large quantity of glutaminic acid, have beensubjected in a well known manner to hydrolysis, for time withhydrochloric specification of Letters Patent.

".by centrifualizin it.

Patented rat. a, rat

application filed December 8, 1916. Serial No. 135,846.

acid, the acid solution is filtered while still hot and-is neutralizedwith sodium carbonate, sodium bicarbonate, sodium hydroxid, calciumhydroxid or calcium carbonate. in short, with the hydroxids of alkalimetals or alkaline earths or with the carbonates of alkali metals oralkaline earths, just enough that only the free hydrochloric acid andthat combined with the amino acids is affectedseparated as thoroughly aspossible from the liquid either by filtering or by centrifugal action.In order to purify the raw glutaminic acid thus obtained the residue isdissolved with a sufiicient quantity of hot I water whereby the saidresinous substance remains behind. This is filtered ofi. There upon somedecolorizing charcoal is added, the solution is evaporated down to abouta third and then filtered while hot, where upon when it is cold the pure.glutaminic acid is precipitated in the form of fine crystals, which areseparated from the motherlye by filtration. This mother-lye when furtherevaporated yields further crystals.

The mother-lye. obtained by the mentioned neutralization with soda orthe like contains the other amino acids and can be further treated toany desired extent.

Example: 150 liters of hydrochloric acld having a specific gravity of1.19 are'p'oured over 50 kilograms of wheat gluten and then heated in aclosed vessel inthe waterbath for approximately 10 hours. The solutlonis then filtered and the filtrate gradually mixed with approximately 55kilograms of calcined soda. The liquid is then cooled and after somedays filtered;

The residue is dissolved in 50.0 liters of hot water andthis solutionimmediatel filtered. Then decolorizing charcoal (accor mg to itsdecolorizing power) is added while stirring, then filtered andevaporated down to about 200-150 liters. When cold, approximately 10 to12 kilograms of. pure glutamlnic acid in the form of fine crystals aredeposlted, which can be separated from the acid, filtering off themineral acid solution,

acid from other amino acids,

If the evaporation of this mother-lye is continued it will yield furthercrystals.

I claim- 1. The process of'separatin glutaminic acid from other aminoacids, Wiich consists in hydrolyzing albumins with mineral acid,filtering'oif the mineral acid solution, treating the latter with aneutralizin agent for the free and combined minera acid, and separatingthe glutaminic acid.

The process of separatin glutaminic acid from other amino. acids, w ichconsists in hydrolyzing albumins with mineral acid, filtering ofl themineral acid solution, treatingithe latter with alkaline metal hydroxidscient to neutralize the free and combined mineral acid.

3. The process of separatin acid from other amino acids, w

in hydrolyzing albumins with h w ich consists 6. The process ofseparating glutaminic acid from other amino acids, which consists inhydrolyzing albumins with hydrochloric acid, filterin the acid solutionwhile hot,

mixing the filtrate with calcined soda then coolinlg the mixture, andfiltering the latter. 7. he process of separatin glutaminic acid fromother amino acids, w ich consists in hydrolyzing albumins withhydrochloric acid, filterin Y mixing the fi ltrate with calcined soda,then cooling the mixture, filtering the latter, dissolving the residuein hot water, filterin the resultant solution, charcoal into the latter,filtering the decolorized solution, evaporating the latter, cooling thesame to obtain crystallization, and separatin the crystals from themother lye by centri ugatio 8. A process of separating glutaminic acidfrom other amino acids, which consists in treating the mineral acidsolution of (glutaminic and other amino acids (obtaine b hydrolysis ofalbumins with mineral acidsl with a compound having an alkaline reactionin quantity suflicient only to neutralize the h drochloric acid presentin the free or combmed state.

In testimony that I claim the foregoing as my invention, I have signedmy name in presence of two subscribing wltnesses.

ARNOLD CORTI;

Witnesses:

CARI. Gunner, OLGA M. Onmc.

the acid solution while hot,

stirring a decolorizing

